Part:BBa_K2272019
pVHb-HAO-Cyt
Device with VHb promter. For specific characterization of the HAO-Cyt coding sequence combination, refer to pBAD-HAO-Cyt(https://parts.igem.org/Part:BBa_K2272020). For characterization of pVHb, refer to https://parts.igem.org/Part:BBa_K2272000.
Usage and Biology
An improved version of https://parts.igem.org/Part:BBa_K1067002 and https://parts.igem.org/Part:BBa_K1067004 by combining the two coding sequences, which were suppose to work hand in hand, together. The VHb promoter was inserted to regulate the nitrification circuit expression under certain hypoxic conditions usually found in Sewage tanks. The homolog developed by DTU-Denmark used what we believed to be an improper coding sequence for a one of the subunits, HAO-B. HAO-B is a putative protein, that at the time may not have been believed to be significant. However, after reviewing the sequence registered in the BLAST database, we believed that HAO-B played a fundamental role in the mechanism of HAO. Since the two parts work hand in hand to nitrify ammonium, we improved the parts from DTU-Denmark by combining the two parts, inserting condionally inducible promoters, and using signal peptides to transport the enzymes to their designated location in the cell.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 484
Illegal XhoI site found at 2048
Illegal XhoI site found at 4257 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 500
Illegal NgoMIV site found at 977
Illegal NgoMIV site found at 981
Illegal NgoMIV site found at 2749
Illegal NgoMIV site found at 3139
Illegal NgoMIV site found at 3406
Illegal NgoMIV site found at 3454 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 404
Illegal BsaI.rc site found at 452
Illegal BsaI.rc site found at 1019
Illegal BsaI.rc site found at 3179
Illegal BsaI.rc site found at 4856
Illegal BsaI.rc site found at 5012
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