Coding

Part:BBa_K2272004

Designed by: Eric Joshua Wang   Group: iGEM17_Virginia   (2017-10-22)


pBAD P.D. TAT(twin-arginine translocation)

This sequence from Paracoccus denitrificans codes for a signal peptide that will export the tagged protein to the periplasm of the cell. The promoter, pBAD (BBa_K731250), that precedes the coding sequence is inducible by arabinose.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 125
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 65
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 171
    Illegal NgoMIV site found at 219
    Illegal NgoMIV site found at 225
    Illegal NgoMIV site found at 240
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None