Part:BBa_K2267028

GFP we Codon optimization Part:BBa_K319039 in G.xylinus

we did point mutation on https://parts.igem.org/Part:BBa_R0062 and the exact spots are

plux1: https://parts.igem.org/Part:BBa_K2267029

plux3: https://parts.igem.org/Part:BBa_K2267030

plux4: https://parts.igem.org/Part:BBa_K2267031

plux6: https://parts.igem.org/Part:BBa_K2267032

plux8: https://parts.igem.org/Part:BBa_K2267046

listed below. We linked gfp reporter genes luxr-plux1-gfp: https://parts.igem.org/Part:BBa_K2267043

luxr-plux3-gfp: https://parts.igem.org/Part:BBa_K2267041

luxr-plux4-gfp: https://parts.igem.org/Part:BBa_K2267042

luxr-plux6-gfp: https://parts.igem.org/Part:BBa_K2267044

luxr-plux8-gfp: https://parts.igem.org/Part:BBa_K2267045

on them respectively to test the function of modified promoters.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 664