Part:BBa_K2252002

FRET Donor

The electron donor component of the FRET made up nowGFP and a gold binding protein

Usage and Biology

Part Transformation results:
Total number of Transformations = 5
Successful number of Transformations = 2
Number of failed transformations = 3

Part gel runs:
Total number of gels runs = 3
Successful number of gel runs = 0
Number of failed gel runs = 3

EXPERIMENT TO DETERMINE THE EFFICIENCY OF THE FRET-DONOR

From figure 4 and 3 above, it is observed that the absorption and fluorescence in the sample increased over time as the bacteria growth increased, however the change was not significant and this concludes that the FRET-Donor is not as effective as expected. This could however be attributed to the fact that concentration of the Donor DNA isn’t enough to produce the expected high absorption and fluorescence values.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 46
  Illegal BamHI site found at 734
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]