Part:BBa_K2252000
pH resistance
Gene conferring pH resistance so E coli can grow at lower pH values
Usage and Biology
INFORMATION ABOUT THE PH RESISTANCE GENE:
Total number of Transformations = 3,
Successful number of Transformations = 2,
Number of failed transformations = 1
Part gel runs:
Total number of gels runs = 3,
Successful number of gel runs = 1,
Number of failed gel runs = 2
GEL IMAGE:
Figure 1 Image of successful gel run
From the gel image in figure 1 above it is evident that the pH resistance gene was inserted in the plasmid, however due to a small concentration of the DNA present, the band of the insert seemed to be blurred out.
EXPERIMENT TO DETERMINE THE EFFICIENCY OF THE pH RESISTANCE GENE
Figure 2 Graph of bacterial growth in different pH levels over a period of time
From the results obtained and displayed in the graph above, it is evident that the pH resistance gene is effective for pH levels ranging from 5.5 to 7.5, where 7.5 is the pH level of the original media for the bacterial growth. It is also observed that, the resistance gene was not effective with pH levels 4.5 and below, resulting in no bacterial growth over time.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 108
- 1000COMPATIBLE WITH RFC[1000]
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