Composite

Part:BBa_K225003

Designed by: Nasa SAVORY   Group: iGEM09_Tokyo-Nokogen   (2009-10-15)

RTC counter element 2

This part consists of crRBS-T7RNA polymerase-Terminator-T7 promoter-crRBS. This part is used for counter system of ESCAPEs system.

MODEL SYSTEM : a model system is constructed by adding high promoter part upstream and GFP-terminator part downstream to this counter2 part. This model part will work by cotransforming counter1(BBa_K225002) model part and this counter2 model part, and arabinose induction. By this induction, taRNA of counter1 model part is transcribed and this transcription product binds to crRBS. After that, T7 RNA polymerase is expressed and binds to T7 promoter. And then, GFP is expressed.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
n/acrRBS-T7RNA polymerase-Terminator-T7 promoter-crRBS