Composite

Part:BBa_K225002

Designed by: Nasa SAVORY   Group: iGEM09_Tokyo-Nokogen   (2009-10-15)

RTC counter element 1

This part consists of RBS-taRNA-Terminator-high promoter-crRBS. This part is used for counter system of ESCAPEs system.

MODEL SYSTEM : a model system is constructed by adding PBAD/ part upstream and RFP-terminator part downstream to this counter1 part. This model part will work by arabinose induction. By this induction, taRNA is transcribed and this transcription product binds to crRBS downstream. And then, RFP is expressed.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 266
    Illegal NheI site found at 289
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 18


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Parameters
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