Part:BBa_K2249000

(EutC1-19)-FusionRed-LVA

This part is a fluorescent protein tagged with two peptide sequences: first, a EutC 1-19 tag that causes it to be sequestered inside EutS compartments (formed with BBa_K2129001 made by CU-Boulder 2016 team). Additionally it has a LVA protein degradation tag. Fluorescent microscopy confirmed that this tag causes degradation of the protein without EutS compartment expression, but does not degrade the fluorescent proteins inside the formed compartments.

Note that without co-expression of EutS (BBa_K2129001), this protein will not be visible. The LVA tag results in very rapid degradation: only being sequestered in a EutS compartment via this part's EutC 1-19 tag will enable visualization.

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FusionRed sequestered inside EutS compartments. Note that contrast and brightness have been optimized to show cell outline in addition to visible compartments.

3D imaging of FusionRed-LVA in EutS compartments taken by the 2017 Bielefeld-CiBiTec iGEM team can be seen here:

"https://static.igem.org/mediawiki/2017/7/75/T-CU_Boulder--EutSCoVideo.mp4"

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 583
  Illegal BsaI.rc site found at 682