Composite

Part:BBa_K2248003

Designed by: Judy Nguyen   Group: iGEM17_Cadets2Vets   (2017-09-17)


C2V_ArsenicCircuit

C2V_ArsenicCircuit is Cadets2Vets arsenic detection circuit. It is a 2-part circuit that combines 2 series of promoter-gene sequences on one plasmid.

The first part of the circuit (BBa_K2248001; PcArsR) contains an E. coli-derived constitutive promoter (Pc), Ribosome Binding Site, Arsenic Regulator (ArsR), and Double Terminator. This sequence allows unregulated expression of ArsR, which is a protein that will change conformation upon the binding of arsenic ions.

The second part of the circuit (BBa_K2248002; PArsRGFP) contains an E. coli-derived Arsenic Regulator promoter (PArsR), Ribosome Binding Site, Green Fluorescent Protein reporter (GFP), and Double Terminator. PArsR sequence has a binding site for ArsR. ArsR is a negative repressor and will release its transcriptional regulation in the presence of arsenic ions. The GFP reporter will turn on and fluoresce in the presence of arsenic.

The two parts were joined together using a SalI Restriction Enzyme Site.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 198
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1319


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