Composite

Part:BBa_K2236002

Designed by: Brendan Reiter   Group: iGEM17_UNBC-Canada   (2017-10-23)


lac inducible expression system for Staph. Hfq

This part is designed for the expression and purification of the Hfq protein from Staphylococcus aureus. The expression should be carried out in E. coli, as the promoter and RBS are optimized for use in E. coli. Expression machinery includes: f1 origin (K314110), lacI that codes for the lac repressor protein (K314111), a lactose inducible promoter (R0011), and the Elowitz standard RBS (B0034). All expression machinery credits to Washington iGEM 2010.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 126
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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