Generator

Part:BBa_K223063

Designed by: Anusuya Ramasubramanian   Group: iGEM09_Stanford   (2009-10-20)


RBS+BLH

Identical to BBa_K223042 but with RBS (B0034) prior to the sequence.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 21
    Illegal suffix found in sequence at 869
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 21
    Illegal SpeI site found at 870
    Illegal PstI site found at 884
    Illegal NotI site found at 27
    Illegal NotI site found at 877
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 21
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 21
    Illegal suffix found in sequence at 870
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 21
    Illegal XbaI site found at 36
    Illegal SpeI site found at 870
    Illegal PstI site found at 884
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None