Generator

Part:BBa_K223061

Designed by: Anusuya Ramasubramanian   Group: iGEM09_Stanford   (2009-10-18)


hIL-6 + HlyAs Fusion protein (Freiburg Compatible)

This sequence when coupled with TolC (BBa_K223055), Front Flanking (BBa_K223056) and Rear Flanking (BBa_K223057) sequences, allows for the extracellular export of hIL-6. This particular part only forms the signal sequence for export.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 867
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
    Illegal NheI site found at 558
    Illegal SpeI site found at 868
    Illegal PstI site found at 882
    Illegal NotI site found at 7
    Illegal NotI site found at 875
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 868
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 1
    Illegal XbaI site found at 16
    Illegal SpeI site found at 868
    Illegal PstI site found at 882
    Illegal AgeI site found at 125
    Illegal AgeI site found at 390
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 249
    Illegal SapI site found at 663


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Categories
Parameters
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