Reporter
Part:BBa_K223060
Designed by: Anusuya Ramasubramanian Group: iGEM09_Stanford (2009-10-18)
5MT Inverter-GFP Reporter and Lac Promoter
Identical to part BBa_K223059 except with a Lac promoter. This part can be used to test the efficiency of the 5MT inverter system.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal prefix found in sequence at 742
Illegal suffix found in sequence at 576
Illegal SpeI site found at 891
Illegal PstI site found at 905 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 229
Illegal EcoRI site found at 742
Illegal SpeI site found at 577
Illegal SpeI site found at 891
Illegal PstI site found at 591
Illegal PstI site found at 905
Illegal NotI site found at 235
Illegal NotI site found at 584
Illegal NotI site found at 748
Illegal NotI site found at 898 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 229
Illegal EcoRI site found at 742 - 23INCOMPATIBLE WITH RFC[23]Illegal prefix found in sequence at 229
Illegal suffix found in sequence at 577
Illegal EcoRI site found at 742
Illegal XbaI site found at 757
Illegal SpeI site found at 891
Illegal PstI site found at 905 - 25INCOMPATIBLE WITH RFC[25]Illegal prefix found in sequence at 229
Illegal EcoRI site found at 742
Illegal XbaI site found at 244
Illegal XbaI site found at 757
Illegal SpeI site found at 577
Illegal SpeI site found at 891
Illegal PstI site found at 591
Illegal PstI site found at 905 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1580
Illegal SapI.rc site found at 423
[edit]
Categories
Parameters
None |