Reporter

Part:BBa_K223059

Designed by: Anusuya Ramasubramanian   Group: iGEM09_Stanford   (2009-10-18)


mutTrp (5MT) Inverter with GFP Biobrick part

A modification to Biobrick part BBa_K223058. It has a GFP biobrick part, BBa_I13504, at the end of the sequence and functions as a reporter.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 534
    Illegal suffix found in sequence at 368
    Illegal SpeI site found at 683
    Illegal PstI site found at 697
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 21
    Illegal EcoRI site found at 534
    Illegal SpeI site found at 369
    Illegal SpeI site found at 683
    Illegal PstI site found at 383
    Illegal PstI site found at 697
    Illegal NotI site found at 27
    Illegal NotI site found at 376
    Illegal NotI site found at 540
    Illegal NotI site found at 690
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 21
    Illegal EcoRI site found at 534
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 21
    Illegal suffix found in sequence at 369
    Illegal EcoRI site found at 534
    Illegal XbaI site found at 549
    Illegal SpeI site found at 683
    Illegal PstI site found at 697
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 21
    Illegal EcoRI site found at 534
    Illegal XbaI site found at 36
    Illegal XbaI site found at 549
    Illegal SpeI site found at 369
    Illegal SpeI site found at 683
    Illegal PstI site found at 383
    Illegal PstI site found at 697
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1372
    Illegal SapI.rc site found at 215


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Categories
Parameters
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