Generator
Part:BBa_K223056
Designed by: Anusuya Ramasubramanian Group: iGEM09_Stanford (2009-10-18)
Front Flanking Sequence (HlyR, IS1, HlyC)
This front flanking sequence has three of the hemolysin parts - HlyR, Is1 and HlyC. In conjunction with rear flanking sequence, containing HlyB and HlyD, and TolC (part K223055), this part creates a channel protein to facilitate the extracellular export HlyAs-fused proteins.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal prefix found in sequence at 1
Illegal suffix found in sequence at 3049
Illegal EcoRI site found at 23
Illegal SpeI site found at 783
Illegal SpeI site found at 795 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1
Illegal EcoRI site found at 23
Illegal SpeI site found at 783
Illegal SpeI site found at 795
Illegal SpeI site found at 3050
Illegal PstI site found at 3064
Illegal NotI site found at 7
Illegal NotI site found at 3057 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1
Illegal EcoRI site found at 23
Illegal BamHI site found at 2887 - 23INCOMPATIBLE WITH RFC[23]Illegal prefix found in sequence at 1
Illegal suffix found in sequence at 3050
Illegal EcoRI site found at 23
Illegal SpeI site found at 783
Illegal SpeI site found at 795 - 25INCOMPATIBLE WITH RFC[25]Illegal prefix found in sequence at 1
Illegal EcoRI site found at 23
Illegal XbaI site found at 16
Illegal SpeI site found at 783
Illegal SpeI site found at 795
Illegal SpeI site found at 3050
Illegal PstI site found at 3064 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 205
Illegal BsaI site found at 906
[edit]
Categories
Parameters
None |