Generator

Part:BBa_K223056

Designed by: Anusuya Ramasubramanian   Group: iGEM09_Stanford   (2009-10-18)


Front Flanking Sequence (HlyR, IS1, HlyC)

This front flanking sequence has three of the hemolysin parts - HlyR, Is1 and HlyC. In conjunction with rear flanking sequence, containing HlyB and HlyD, and TolC (part K223055), this part creates a channel protein to facilitate the extracellular export HlyAs-fused proteins.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 3049
    Illegal EcoRI site found at 23
    Illegal SpeI site found at 783
    Illegal SpeI site found at 795
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
    Illegal EcoRI site found at 23
    Illegal SpeI site found at 783
    Illegal SpeI site found at 795
    Illegal SpeI site found at 3050
    Illegal PstI site found at 3064
    Illegal NotI site found at 7
    Illegal NotI site found at 3057
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1
    Illegal EcoRI site found at 23
    Illegal BamHI site found at 2887
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 3050
    Illegal EcoRI site found at 23
    Illegal SpeI site found at 783
    Illegal SpeI site found at 795
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 1
    Illegal EcoRI site found at 23
    Illegal XbaI site found at 16
    Illegal SpeI site found at 783
    Illegal SpeI site found at 795
    Illegal SpeI site found at 3050
    Illegal PstI site found at 3064
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 205
    Illegal BsaI site found at 906


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Categories
Parameters
None