Generator
Part:BBa_K223052
Designed by: Anusuya Ramasubramanian Group: iGEM09_Stanford (2009-10-18)
5MT Mutant TrpR gene
The 5 methyl-L-tryptophan aporepressor gene is a mutant version of the regular TrpR gene involved in sensing levels of synthetic compound, 5 MT. It works by binding to its corepressor, 5 MT, and then to its corresponding promoter operator, BBa_K223051, repressing downstream transcription. In the presence of low corepressor levels, the 5MT aporepressor will not bind allowing for downstream transcription.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal prefix found in sequence at 1
Illegal suffix found in sequence at 348 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1
Illegal SpeI site found at 349
Illegal PstI site found at 363
Illegal NotI site found at 7
Illegal NotI site found at 356 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1
- 23INCOMPATIBLE WITH RFC[23]Illegal prefix found in sequence at 1
Illegal suffix found in sequence at 349 - 25INCOMPATIBLE WITH RFC[25]Illegal prefix found in sequence at 1
Illegal XbaI site found at 16
Illegal SpeI site found at 349
Illegal PstI site found at 363 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 195
[edit]
Categories
Parameters
n/a | 5MT Mutant TrpR gene |