Generator

Part:BBa_K223052

Designed by: Anusuya Ramasubramanian   Group: iGEM09_Stanford   (2009-10-18)


5MT Mutant TrpR gene

The 5 methyl-L-tryptophan aporepressor gene is a mutant version of the regular TrpR gene involved in sensing levels of synthetic compound, 5 MT. It works by binding to its corepressor, 5 MT, and then to its corresponding promoter operator, BBa_K223051, repressing downstream transcription. In the presence of low corepressor levels, the 5MT aporepressor will not bind allowing for downstream transcription.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 348
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
    Illegal SpeI site found at 349
    Illegal PstI site found at 363
    Illegal NotI site found at 7
    Illegal NotI site found at 356
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 349
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 1
    Illegal XbaI site found at 16
    Illegal SpeI site found at 349
    Illegal PstI site found at 363
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 195


[edit]
Categories
Parameters
n/a5MT Mutant TrpR gene