Regulatory
Part:BBa_K223051
Designed by: Anusuya Ramasubramanian Group: iGEM09_Stanford (2009-10-18)
5MT operon (promoter-operator)
This is a modified trp operon, promoter and operator site, that recognizes and binds to a doubly mutant repressor and its corepressor, 5-methyl-L-tryptophan. In conjunction with the mutant tryptophan repressor, this modified operon functions as an inverter, converting decreasing levels of 5MT into a PoPs output signal for downstream transcription.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal prefix found in sequence at 1
Illegal suffix found in sequence at 149 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1
Illegal SpeI site found at 150
Illegal PstI site found at 164
Illegal NotI site found at 7
Illegal NotI site found at 157 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1
- 23INCOMPATIBLE WITH RFC[23]Illegal prefix found in sequence at 1
Illegal suffix found in sequence at 150 - 25INCOMPATIBLE WITH RFC[25]Illegal prefix found in sequence at 1
Illegal XbaI site found at 16
Illegal SpeI site found at 150
Illegal PstI site found at 164 - 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
n/a | 5MT operon (promoter-operator) |