Regulatory

Part:BBa_K223051

Designed by: Anusuya Ramasubramanian   Group: iGEM09_Stanford   (2009-10-18)

5MT operon (promoter-operator)

This is a modified trp operon, promoter and operator site, that recognizes and binds to a doubly mutant repressor and its corepressor, 5-methyl-L-tryptophan. In conjunction with the mutant tryptophan repressor, this modified operon functions as an inverter, converting decreasing levels of 5MT into a PoPs output signal for downstream transcription.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 149
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
    Illegal SpeI site found at 150
    Illegal PstI site found at 164
    Illegal NotI site found at 7
    Illegal NotI site found at 157
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 150
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 1
    Illegal XbaI site found at 16
    Illegal SpeI site found at 150
    Illegal PstI site found at 164
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
n/a5MT operon (promoter-operator)