Generator

Part:BBa_K223050

Designed by: Suzanne Bartram   Group: iGEM09_Stanford   (2009-10-17)


SoxR - SoxS System with Blh and RALDH II system and Lac promoter

Same as BBa_K223049 with the addition of an inducible lacl promoter.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 880
    Illegal suffix found in sequence at 714
    Illegal EcoRI site found at 1013
    Illegal EcoRI site found at 1910
    Illegal XbaI site found at 1028
    Illegal XbaI site found at 1925
    Illegal SpeI site found at 965
    Illegal SpeI site found at 1862
    Illegal SpeI site found at 3469
    Illegal PstI site found at 979
    Illegal PstI site found at 1876
    Illegal PstI site found at 3483
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 229
    Illegal EcoRI site found at 880
    Illegal EcoRI site found at 1013
    Illegal EcoRI site found at 1910
    Illegal SpeI site found at 715
    Illegal SpeI site found at 965
    Illegal SpeI site found at 1862
    Illegal SpeI site found at 3469
    Illegal PstI site found at 729
    Illegal PstI site found at 979
    Illegal PstI site found at 1876
    Illegal PstI site found at 3483
    Illegal NotI site found at 235
    Illegal NotI site found at 722
    Illegal NotI site found at 886
    Illegal NotI site found at 972
    Illegal NotI site found at 1019
    Illegal NotI site found at 1869
    Illegal NotI site found at 1916
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 229
    Illegal EcoRI site found at 880
    Illegal EcoRI site found at 1013
    Illegal EcoRI site found at 1910
    Illegal BglII site found at 2026
    Illegal BglII site found at 3160
    Illegal XhoI site found at 2007
    Illegal XhoI site found at 3063
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 229
    Illegal suffix found in sequence at 715
    Illegal EcoRI site found at 880
    Illegal EcoRI site found at 1013
    Illegal EcoRI site found at 1910
    Illegal XbaI site found at 895
    Illegal XbaI site found at 1028
    Illegal XbaI site found at 1925
    Illegal SpeI site found at 965
    Illegal SpeI site found at 1862
    Illegal SpeI site found at 3469
    Illegal PstI site found at 979
    Illegal PstI site found at 1876
    Illegal PstI site found at 3483
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 229
    Illegal EcoRI site found at 880
    Illegal EcoRI site found at 1013
    Illegal EcoRI site found at 1910
    Illegal XbaI site found at 244
    Illegal XbaI site found at 895
    Illegal XbaI site found at 1028
    Illegal XbaI site found at 1925
    Illegal SpeI site found at 715
    Illegal SpeI site found at 965
    Illegal SpeI site found at 1862
    Illegal SpeI site found at 3469
    Illegal PstI site found at 729
    Illegal PstI site found at 979
    Illegal PstI site found at 1876
    Illegal PstI site found at 3483
    Illegal NgoMIV site found at 2540
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2878


[edit]
Categories
Parameters
n/aSoxR - SoxS System with Blh and RALDH II system and Lac promoter