Generator

Part:BBa_K223049

Designed by: Suzanne Bartram   Group: iGEM09_Stanford   (2009-10-17)


SoxR - SoxS System with Blh and RALDH II system

This is the superoxide inducible SoxR-SoxS promoter system coupled with the production of Blh and RALDH II genes for the cleaving of beta carotene into retinoic acid.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 672
    Illegal suffix found in sequence at 506
    Illegal EcoRI site found at 805
    Illegal EcoRI site found at 1702
    Illegal XbaI site found at 820
    Illegal XbaI site found at 1717
    Illegal SpeI site found at 757
    Illegal SpeI site found at 1654
    Illegal SpeI site found at 3261
    Illegal PstI site found at 771
    Illegal PstI site found at 1668
    Illegal PstI site found at 3275
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 21
    Illegal EcoRI site found at 672
    Illegal EcoRI site found at 805
    Illegal EcoRI site found at 1702
    Illegal SpeI site found at 507
    Illegal SpeI site found at 757
    Illegal SpeI site found at 1654
    Illegal SpeI site found at 3261
    Illegal PstI site found at 521
    Illegal PstI site found at 771
    Illegal PstI site found at 1668
    Illegal PstI site found at 3275
    Illegal NotI site found at 27
    Illegal NotI site found at 514
    Illegal NotI site found at 678
    Illegal NotI site found at 764
    Illegal NotI site found at 811
    Illegal NotI site found at 1661
    Illegal NotI site found at 1708
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 21
    Illegal EcoRI site found at 672
    Illegal EcoRI site found at 805
    Illegal EcoRI site found at 1702
    Illegal BglII site found at 1818
    Illegal BglII site found at 2952
    Illegal XhoI site found at 1799
    Illegal XhoI site found at 2855
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 21
    Illegal suffix found in sequence at 507
    Illegal EcoRI site found at 672
    Illegal EcoRI site found at 805
    Illegal EcoRI site found at 1702
    Illegal XbaI site found at 687
    Illegal XbaI site found at 820
    Illegal XbaI site found at 1717
    Illegal SpeI site found at 757
    Illegal SpeI site found at 1654
    Illegal SpeI site found at 3261
    Illegal PstI site found at 771
    Illegal PstI site found at 1668
    Illegal PstI site found at 3275
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 21
    Illegal EcoRI site found at 672
    Illegal EcoRI site found at 805
    Illegal EcoRI site found at 1702
    Illegal XbaI site found at 36
    Illegal XbaI site found at 687
    Illegal XbaI site found at 820
    Illegal XbaI site found at 1717
    Illegal SpeI site found at 507
    Illegal SpeI site found at 757
    Illegal SpeI site found at 1654
    Illegal SpeI site found at 3261
    Illegal PstI site found at 521
    Illegal PstI site found at 771
    Illegal PstI site found at 1668
    Illegal PstI site found at 3275
    Illegal NgoMIV site found at 2332
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2670


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Categories
Parameters
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