Part:BBa_K223048

Blh- RALDH II Coupled Generator

This is the RBS- Blh- RBS- RALDH II- TT generator. Both of these parts are important in the cleaving of beta carotene into retinoic acid.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal prefix found in sequence at 918
  Illegal suffix found in sequence at 869
  Illegal SpeI site found at 2477
  Illegal PstI site found at 2491
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 21
  Illegal EcoRI site found at 918
  Illegal SpeI site found at 870
  Illegal SpeI site found at 2477
  Illegal PstI site found at 884
  Illegal PstI site found at 2491
  Illegal NotI site found at 27
  Illegal NotI site found at 877
  Illegal NotI site found at 924
  Illegal NotI site found at 2484
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 21
  Illegal EcoRI site found at 918
  Illegal BglII site found at 1034
  Illegal BglII site found at 2168
  Illegal XhoI site found at 1015
  Illegal XhoI site found at 2071
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal prefix found in sequence at 21
  Illegal suffix found in sequence at 870
  Illegal EcoRI site found at 918
  Illegal XbaI site found at 933
  Illegal SpeI site found at 2477
  Illegal PstI site found at 2491
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal prefix found in sequence at 21
  Illegal EcoRI site found at 918
  Illegal XbaI site found at 36
  Illegal XbaI site found at 933
  Illegal SpeI site found at 870
  Illegal SpeI site found at 2477
  Illegal PstI site found at 884
  Illegal PstI site found at 2491
  Illegal NgoMIV site found at 1548
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 1886