Reporter

Part:BBa_K223046

Designed by: Suzanne Bartram   Group: iGEM09_Stanford   (2009-10-17)


SoxR - SoxS - GFP Reporter

SoxR - SoxS Promoter System with downstream GFP production. This was used to characterize the inducible oxidative stress signal when activating the SoxR-SoxS promoter system.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 672
    Illegal suffix found in sequence at 506
    Illegal SpeI site found at 757
    Illegal PstI site found at 771
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 21
    Illegal EcoRI site found at 672
    Illegal SpeI site found at 507
    Illegal SpeI site found at 757
    Illegal PstI site found at 521
    Illegal PstI site found at 771
    Illegal NotI site found at 27
    Illegal NotI site found at 514
    Illegal NotI site found at 678
    Illegal NotI site found at 764
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 21
    Illegal EcoRI site found at 672
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 21
    Illegal suffix found in sequence at 507
    Illegal EcoRI site found at 672
    Illegal XbaI site found at 687
    Illegal SpeI site found at 757
    Illegal PstI site found at 771
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 21
    Illegal EcoRI site found at 672
    Illegal XbaI site found at 36
    Illegal XbaI site found at 687
    Illegal SpeI site found at 507
    Illegal SpeI site found at 757
    Illegal PstI site found at 521
    Illegal PstI site found at 771
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1446


[edit]
Categories
Parameters
n/aSoxR - SoxS - GFP Reporter