Generator

Part:BBa_K223040

Designed by: Suzanne Bartram   Group: iGEM09_Stanford   (2009-10-17)


SoxR Gene: Produces the SoxR protein

This part produces the SoxR protein, which in the presence of oxidative stresses, such as paraquat and nitric oxide, binds with high affinity to the SoxS promoter. The SoxR generator, when coupled with the SoxS promoter, can be utilized as an inducible sensor for oxidative stress.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 486
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
    Illegal SpeI site found at 487
    Illegal PstI site found at 501
    Illegal NotI site found at 7
    Illegal NotI site found at 494
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 487
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 1
    Illegal XbaI site found at 16
    Illegal SpeI site found at 487
    Illegal PstI site found at 501
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
n/aSoxR Gene: Produces the SoxR protein