Coding

Part:BBa_K2224007

Designed by: Yunchang Zhang   Group: iGEM17_SMS_Shenzhen   (2017-10-26)


Lecanicillium lecanii strain CA-12 cuticle-degrading proteinase (Pr1)

This part is a proteinase, which is from the Lecanicillium lecanii strain CA-12. The Lecanicillium lecanii uses this proteinase to degrade the protein-chitin shell of scale insect. This part can be transformed into E.coli to express proteinase, which shows a strong ability in degrading the protein layer under the Scanning Electron Microscope. In SMS_Shenzhen 2017 project, this proteinase functions as a core mechanism to degrade the chitin-protein complex,in which the protein wraps up the chitin to form the hard layer.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 60
    Illegal AgeI site found at 421
    Illegal AgeI site found at 1087
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None