Composite

Part:BBa_K2224001

Designed by: Yunchang Zhang   Group: iGEM17_SMS_Shenzhen   (2017-10-24)


Promoter+RBS+Lecanicillium lecanii strain 432 endochitinase (chit1)+Terminator

This composite part contains a coding sequence of chitinase, which is from the Lecanicillium lecanii strain 432. The Lecanicillium lecanii uses this chitinase to degrade the shell of scale insect. This part can be transformed into E.coli to express chitinase, which shows a strong ability in degrading chintin into N-acetylglucosamine. In SMS_Shenzhen_2017 project, this chintinase functions as a core mechanism to degrade the chitin-protein complex, which makes up the second layer of the shell of scale insect.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 218
    Illegal NgoMIV site found at 677
    Illegal NgoMIV site found at 884
    Illegal AgeI site found at 786
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None