Reporter

Part:BBa_K2223005

Designed by: Thomas Sousa   Group: iGEM17_UCSC   (2017-11-01)


B_12 Riboswitch reporter

The riboswitch part contains a psbAI promoter which transcribes an mRNA strand consisting of a riboswitch and repressor gene, TetR. The riboswitch binds to vitamin B12 and regulates the expression of the downstream repressor gene. In the presence of vitamin B12, the riboswitch specifically anneals onto the cobalamin molecule inducing a conformational change within the mRNA sequestering the riboswitch binding site upstream of TetR. However, in the absence of vitamin B12, TetR is continuously expressed.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 631
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 631
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 631
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 631
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None