Coding

Part:BBa_K2223003

Designed by: Thomas Sousa   Group: iGEM17_UCSC   (2017-11-01)


bluB

The gene bluB encodes an enzyme that catalyzes an oxidized reaction that cleaves the reduced Flavin mononucleotide (FMNH2) transforming to 5,6-dimethylbenzimidazole (5,6-DMB). The bluB sequence was optimized for PCC 7942 using the reference genome sequence from Sinorhizobium meliloti 1021. bluB --> 5,6-DMB synthase

Organism: Sinorhizobium meliloti 1021

Biosafety Level I


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 680
    Illegal NheI site found at 684
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 648
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None