Coding

Part:BBa_K2223002

Designed by: Pratibha Jagannatha   Group: iGEM17_UCSC   (2017-10-27)


ssuE

The enzyme created by the gene ssuE catalyzes activity that reduces the Flavin mononucleotide (FMN) by using NADH dehydrogenase as a reducing agent. This gene sequenced was optimized from S. elonagtus PCC 7002 sequence.

Original Organism: Synechoccocous elongatus strain PCC 7002
Biosafety Level I

The synthesis of 5,6-DMB first requires the presence of reduced flavin mononucleotide (FMNH2). FMNH2 is reduced from flavin mononucleotide (FMN) by FMN reductase, which is encoded with the ssuE gene.

FMNH2 + NADP+ = FMN + NADPH


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 111
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
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