GlmY with a constitutive promoter
Updated: Fall 2018
Group: Gaston_Day_School, 2018
Author: Carlos Sanchez
Summary: A series of tests examining isobutanol resistance were run and the data compared to the resistance of a control strain. The data show that there is a significant reduction in isobutanol resistance in K2221002 (* p<0.05).
GlmY is involved in cell membrane structure as well as alcohol resistance. We think that the level of overexpression from the high efficiency promoter J23102 causes the cells to be more sensitive to environmental stress than control cells.
Figure 1: GlmY with a constitutive promoter
Gel showing our working GlmY + J23102 construct Wells are as follows: 1.ladder 2.Control 3. J23102 4. GlmY 5. GlmY 6. GlmY + J23102 7. GlmY + J23102 8. GlmY + J23102 Sequence and Features
- 10COMPATIBLE WITH RFC
- 12Illegal NheI site found at 7
Illegal NheI site found at 30
- 21COMPATIBLE WITH RFC
- 23COMPATIBLE WITH RFC
- 25COMPATIBLE WITH RFC
- 1000COMPATIBLE WITH RFC