Coding

Part:BBa_K2221000

Designed by: Haina Saqibo   Group: iGEM17_Gaston_Day_School   (2017-10-26)


GlmY RNA activates GlmZ

Updated: Fall 2018

Group: Gaston_Day_School, 2018

Author: Carlos Sanchez

Summary: Initial data suggested that there was an increase in isobutanol resistance with GlmY alone. Further testing and statistical analysis have shown that there is no change in resistance (27.5 mM vs 31.5 mM).


GlmYcontrol.png

GlmY RNA activates GlmZ to upregulate the synthesis of GlmS.

%E5%BE%AE%E4%BF%A1%E5%9B%BE%E7%89%87_20171101233447.jpg

Gel showing our working GlmY: construct Wells are as follows: 1.ladder 2.Control 3. J23102 4. GlmY 5. GlmY

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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