Regulatory

Part:BBa_K2209000

Designed by: Qinyu Ge   Group: iGEM17_Nanjing_NFLS   (2017-10-17)


Modified promoter of aniline dioxygenase

The regulating protein coding by R gene of aniline dioxygenase can bind to the promoter which inhibiting the transcription and expression of aniline dioxygenase. The key sequences of the promoter could be random mutated which interfering the binding of regulating protein and stimulating transcription and expression of the gene. Two base of the nucleotide in promoter were mutated in this study.

Characterization of part BBa_K2209000

It is expected that aniline dioxygenase from environment samples could constitutive expression by the modification. Experiment result showed that it is achieved as follows, the gene was expressed whether it is induced.

Pic 1.png


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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