Coding

Part:BBa_K2207036

Designed by: Shisheng Li   Group: iGEM17_ZJU-China   (2017-10-23)


oligoA-mRFP

This part is optimized from BBa_E1010, a really good report gene for iGEMers. However, to express in Saccharomyces cerevisiae BY4741, it must contain the kozac sequence. In Saccharomyces cerevisiae, the kozac sequence can be aaaaaaatg, so we add an oligo adenine nucleotide(6bp) before the start codon of the mRFP.


600px-ZJU-China_2017_IMG_4391.JPG

Left: untrangenic BY4741 Right: mRFP(BBa_K2207036) in GPD-CYC1 system(BBa_K2207018)

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 561
    Illegal AgeI site found at 673
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None