Part:BBa_K2207017

phlCD enzyme

DAPG is initially found in specific strain of Pseudomonas fluorescens. And it has been demonstrated that a cluster named phl responsible for the biosynthesis of DAPG, which contains eight genes, from phlA to phlH. Further studies shows that phlABCD are the DAPG synthesis genes.We successfully cloned the phlABCD via colony PCR from Pseudomonas fluorescens 2P24. In this part, with the M2S intergration technique , whose mechanism is similar to that of Trichoderma Homologous Recombination Kit we providing，the dual transcription unit, L2-tCCW12-phlC-TEF2-PGK1-phlD-tRPL9A-L3-homologous arm， was constructed. It can be cotransformed with BBa_K2207016 to intergrate phlABCD into the chromosome of Saccharomyces cerevisiae.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal XbaI site found at 3244
  Illegal XbaI site found at 4654
  Illegal SpeI site found at 1798
  Illegal SpeI site found at 5081
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal SpeI site found at 1798
  Illegal SpeI site found at 5081
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal XhoI site found at 1399
  Illegal XhoI site found at 4737
  Illegal XhoI site found at 4904
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal XbaI site found at 3244
  Illegal XbaI site found at 4654
  Illegal SpeI site found at 1798
  Illegal SpeI site found at 5081
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal XbaI site found at 3244
  Illegal XbaI site found at 4654
  Illegal SpeI site found at 1798
  Illegal SpeI site found at 5081
  Illegal NgoMIV site found at 2309
  Illegal AgeI site found at 1090
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 3941