Part:BBa_K2207016

phlAB enzyme

DAPG is initially found in specific strain of Pseudomonas fluorescens. And it has been demonstrated that a cluster named phl responsible for the biosynthesis of DAPG, which contains eight genes, from phlA to phlH. Further studies shows that phlABCD are the DAPG synthesis genes.We successfully cloned the phlABCD via colony PCR from Pseudomonas fluorescens 2P24. In this part, with the M2S intergration technique , whose mechanism is similar to that of Trichoderma Homologous Recombination Kit we providing，the dual transcription unit, homologous arm-URA3 marker-L1-tRPS2-phlA-TEF2-PGK1-phlB-tTDH1-L2， was constructed.It can be cotransformed with BBa_K2207017 to intergrate phlABCD into the chromosome of Saccharomyces cerevisiae.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 5143
  Illegal SpeI site found at 3321
  Illegal SpeI site found at 5309
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 5143
  Illegal SpeI site found at 3321
  Illegal SpeI site found at 5309
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 5143
  Illegal XhoI site found at 2678
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 5143
  Illegal SpeI site found at 3321
  Illegal SpeI site found at 5309
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 5143
  Illegal SpeI site found at 3321
  Illegal SpeI site found at 5309
  Illegal NgoMIV site found at 2978
  Illegal NgoMIV site found at 3265
  Illegal NgoMIV site found at 3832
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 1516
  Illegal SapI site found at 414
  Illegal SapI.rc site found at 1363