Part:BBa_K2205028

Sensynova Framework (PSP3 pag signal tuner Processing Module - pCell) - connector 1B ONLY

Description

The part BBa_K2205025 was designed as Processor unit of the Sensynova multicellular framework for biosensors development (Figure 1).

This part is the intermediate part produced in the making of part BBa_K2205025 which was made to test the modularity of the system by inserting the PSP3 Phage Sensitivity Tuner (BBa_K274381) construct between the two parts of the empty processing module of the Sensynova platform.

In this part (BBa_K2205028), the part BBa_ K274381 is under the control of our connector 1, part BBa_K2205010, however, not yet controlling our connector 2, part BBa_K2205011.

When complete, the system will work through the repression of the part BBa_K274381 except in the presence of connector 1 subsequently activating the transcription of connector 2 (C12 – RHL) triggering the reporter modules of the platform.

References

iGEM Community. (2009). Team Cambridge 2009. [online] Available at: http://2009.igem.org/Team:Cambridge [Accessed 30 Oct. 2017].

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 7
  Illegal NheI site found at 30
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 383
  Illegal AgeI site found at 580
  
• 1000
  COMPATIBLE WITH RFC[1000]