Part:BBa_K2205025

Sensynova Framework (PSP3 pag signal tuner Processing Module - pCell)

Description

The part BBa_K2205025 was designed as Processor unit of the Sensynova multicellular framework for biosensors development (Figure 1).

This part was made to test the modularity of the system by inserting the PSP3 Phage Sensitivity Tuner (BBa_K274381) construct between the two parts of the empty processing module of the Sensynova platform.

The part BBa_ K274381 is under the control of our connector 1, part BBa_K2205010, while controlling our connector 2, part BBa_K2205011.

This system is normally repressed except in the presence of connector 1 subsequently activating the transcription of connector 2 (C12 – RHL) triggering the reporter modules of the platform.

References

iGEM Community. (2009). Team Cambridge 2009. [online] Available at: http://2009.igem.org/Team:Cambridge [Accessed 30 Oct. 2017].

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 7
  Illegal NheI site found at 30
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 383
  Illegal AgeI site found at 580
  
• 1000
  COMPATIBLE WITH RFC[1000]