Device

Part:BBa_K2205012

Designed by: Jessica Birt, Declan Kohl   Group: iGEM17_Newcastle   (2017-09-21)


Sensynova Framework (Blank Processing Module - pCell)

The part BBa_K2205012 was designed as Processor unit of the Sensynova multicellular framework for biosensors development(Figure 1).

Framework generic.jpg


In vivo Qualitative Comparison

The connector 1 (C4-HSL) was added to the co-culture consisting of processor + reporter BBa_K2205015 in ratio 1:1. The plot below shows the successful communication via quorum sensing in the Sensynova device. It is clear that the presence of 1mM C4-HSL is detected by the processor cells which produce the connector 2 (C12-RHL) for the reporter cells to detect. This induction in the reporter cells leads to the expression of sfGFP (Figure 2)


Hsl proc rep.jpg

Sequence and Features

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 383
    Illegal AgeI site found at 580
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None