Coding
tRNA

Part:BBa_K2201408

Designed by: Olga Schmidt   Group: iGEM17_Bielefeld-CeBiTec   (2017-10-27)


tyrosyl-tRNA


For incorporation of non-canonical amino acids orthogonal tRNA and aminoacyl-tRNA synthetases are needed. This pair needs to be adapted to recognize and incorporate the the non-canonical amino acid with high specifity. This part contains the tyrosyl-tRNA of Methanococcus jannaschii which is adapted to recognize the amber codon.

Usage and Biology

tRNAs are esterified with amino acids by their corresponding aminoacyl-tRNA synthetases. This part is essential for the selection plasmids to select functioning aminoacyl-tRNA snthetases from the generated library. This tyrosyl-tRNA was adapted to suppress the amber codon allowing the incorporation of non-canonical amino acids at the this particular stop codon.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None