Regulatory

Part:BBa_K220004

Designed by: Sarah Sandock   Group: iGEM09_Wisconsin-Madison   (2009-10-18)

Salt Stress Activated Promoter Pro U

The ProU promoter (658 bp) was obtained from wild type K12 E. Coli Genomic DNA. Pro U controls transcription of the Pro U operon which contains three sub-units instrumental in bringing choline and proline into the cell. The promoter is most active during times of osmotic stress. We PCR amplified the gene proU with iGEM designated cutting sites and ribosome binding sites and biobricked it with pSB1A2.


Reference: T Annamalai, K Venkitanarayanan Role of proP and proU in betaine uptake by Y. enterocolitica under cold and osmotic stress conditions http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2655450/?tool=pubmed"


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None