Coding

Part:BBa_K220003

Designed by: Sarah Sandock   Group: iGEM09_Wisconsin-Madison   (2009-10-18)


ADP - sugar Pyrophosphatase (NudF)

nudF is a plasmid gene (560 bp) that codes for the protein responsible for the enzymatic reaction in mevalonent pathway to form Dimethylallyl pyrophosphate (DMAPP). Specifically, in our project, we used it in the last step of mevalonate pathway to produce biofuels iso-pentenol. We PCR amplified gene nudF with iGEM designated cutting sites and ribosome binding sites and biobricked it with pSB1A2.

Reference: ST Withers and JD Keasling, Biosynthesis and engineering of isoprenoid small molecules, http://www.springerlink.com/content/p118kp4816486248/fulltext.html


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 286
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None