Part:BBa_K220000

Sarcoisine Dimethyl Glycine Methyl Transferase (SDMT)

!!! WARNING: Pst1 cut site within gene !!!

SDMT is a plasmid gene (900 bp) that codes for the enzyme that catalyze the first reaction in the salt tolerant pathway that we are interested. This enzyme of this pathway is SDMT, which stands for Sarcosine Dimethylglycine Methyl Transferase. It catalyzes two steps of methylation, both that of sarcosine and dimethylglycine, to our final product, betaine, again using SAM as the methyl donor. The gene we used came from Galdieria sulphuraria, an extremephile that establishes high resistance to osmotic stress as well as acidic, thermal stresses and toxic metals. In our project, we really appreciate that Center for Eukaryotic Structural Genomics at University of Wisconsin-Madison graciously gave us the plasmid containing this gene. We PCR amplified gene SDMT with iGEM designated cutting sites and ribosome binding sites and biobricked it with pSB1A2.

Modeling of [http://2009.igem.org/Team:Wisconsin-Madison/SDMT SDMT]

Modeling of [http://2009.igem.org/Team:Wisconsin-Madison/GSMT%2BSDMT GSMT and SDMT]

Reference: J McCoy, L Bailey, Y NG, C Bingman, R Wrobel, A Weber, B Fox, G Philips Discovery of sarcosine dimethylglycine methyltransferase from Galdieria sulphuraria http://www3.interscience.wiley.com/cgi-bin/fulltext/120751555/HTMLSTART

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal PstI site found at 777
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal PstI site found at 777
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 71
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal PstI site found at 777
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal PstI site found at 777
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI.rc site found at 836