Composite

Part:BBa_K2197600

Designed by: Ip Wang Chak   Group: iGEM17_Hong_Kong_UCCKE   (2017-10-13)


Expression of Aprotinin under the control of a uric acid concentration-sensitive promoter device

This composite part consists of two sessions which are the promoter and BBa_K1456006 (designed by Mustafa Semih Elitok iGEM14_ATOMS-Turkiye). The promoter HucR, which is a bacterial transcriptional repressor will dissociate from the binding site hucO8 in the presence of uric acid and allow the expression of BBa_K1456006, Aprotinin. Aprotinin is a potent protease inhibitor which prevents the formation of xanthine oxidase (XO) from xanthine dehydrogenase (XDH). XO is an enzyme catalysing the oxidation of hypoxanthine to xanthine and can further catalyze the oxidation of xanthine to uric acid.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 1098
    Illegal NheI site found at 1121
    Illegal NotI site found at 1175
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 555
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1197
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 291
    Illegal SapI.rc site found at 255


[edit]
Categories
Parameters
None