Composite

Part:BBa_K2197510

Designed by: Ip Wang Chak   Group: iGEM17_Hong_Kong_UCCKE   (2017-10-13)


Expression of GLUT9 under the control of a constitutive promoter

This composite part encodes a uric acid and glucose transporter Glut 9b(also known as SLC2A9), a gene originated from homo sapiens. Using the same construct as K2197500, the main gene of interest is swapped to Glut 9b, instead of Ygfu.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1329
    Illegal PstI site found at 1397
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal PstI site found at 1329
    Illegal PstI site found at 1397
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 691
    Illegal BamHI site found at 1159
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1329
    Illegal PstI site found at 1397
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1329
    Illegal PstI site found at 1397
    Illegal NgoMIV site found at 1460
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1259
    Illegal BsaI.rc site found at 1620


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