Part:BBa_K2197502
Expression of YgFU under the control of a uric acid concentration-sensitive promoter device
According to our research, one-third of serum uric acid is found in our alimentary canal(primarily small intestines). By enclosing the engineered bacteria (BBa_K2197502) in a capsule, we hope i to absorb excess uric acid produced in the guts. Our ultimate goal is to create a capsule of transformed E.coli with parts BBa_K2197400 and BBa_K2197502. When the capsule is taken into the human body, E.coli in the capsule absorbs uric acid from the digestive canal with aid with the transporter protein expressed by part BBa_K2197500. After absorbing the uric acid in the digestive canal, part BBa_K2197400 senses the level of uric acid and then secrete different amount of smUOX accordingly. It is hoped that most uric acid is digested by the smUOX secreted.
Design of BBa_K2197502
Operator site and YgFU
Expression of a strong repressor (mUTS)
Expression without uric acid (-UA)
mUTS binds with HucO and repress the expression of downstream YgFU.
Expression with uric acid (+UA)
mUTS dissociates with HucO to different extents according to the concentration of uric acid, thus expressing downstream YgFU at different levels.
This composite part can also be divided into two sessions. The uric acid concentration-sensitive promoter and BBa_K2197500. Referring to the mechanism of the promoter that we have explained in the project design page, when there is a large amount of uric acid, the repressor protein mUTS will disassociate from HucO, the operating site, and allow the expression of downstream gene which will express more Ygfu. The higher expression rate of Ygfu allows more uric acid to be absorbed into the E. coli, so that it can lower the uric acid concentration outside the cells.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal NheI site found at 1098
Illegal NheI site found at 1121 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1439
Illegal XhoI site found at 555 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1621
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 291
Illegal SapI.rc site found at 255
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