Protein_Domain
YgfU

Part:BBa_K2197501

Designed by: Ip Wang Chak   Group: iGEM17_Hong_Kong_UCCKE   (2017-10-13)


E.coli-originated uric acid transporter that absorbs surrounding uric acid into the cell

This basic part encodes an E.coli-originated uric acid transporter that tranports surrounding uric acid(urate) into the cell and allows the urate oxidase to catalyze uric acid into allantoin. According to other research, ygfu is a proton-gradient dependent, low-affinity (Km 0.5 mm), and high-capacity transporter for uric acid.1.)The sequence was obtained from NCBI 3.) and was optimize for IDT Gene Block DNA synthesis


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 269
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 451
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None