Part:BBa_K2197402
Mammalian version of urate oxidase (UOX)
This is a subpart of BBa_K2197400. The part expresses smUOX, a mammalian version of urate oxidase. In the human purine and uric acid pathway, purine is converted to uric acid through a series of steps in the purine pathway. Uric acid forms crystals when high concentration is accumulated. Urate oxidase breaks down uric acid into allantoin so that the crystals can be excreted.
Design of BBa_K2197400
Expression of a strong repressor (mUTS)
Operator site and smUOX
Expression without uric acid (-UA)
mUTS binds to HucO and repress the expression of downstream smUOX.
expression with uric acid (+UA)
mUTS dissociates with HucO to different extents according to the concentration of uric acid, thus expresses downstream smUOX at different levels.
Kemmer, Christian & Gitzinger, Marc & Daoud-El Baba, Marie & Djonov, Valentin & Stelling, Jörg & Fussenegger, Martin. (2010). Self-sufficient control of urate homeostasis in mice by a synthetic circuit. Nature biotechnology. 28. 355-60. 10.1038/nbt.1617.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 643
Illegal PstI site found at 865 - 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 736
Illegal PstI site found at 643
Illegal PstI site found at 865 - 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 643
Illegal PstI site found at 865 - 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 643
Illegal PstI site found at 865 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 4
Illegal SapI.rc site found at 954
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