Protein_Domain
smUOX

Part:BBa_K2197402

Designed by: Ip Wang Chak   Group: iGEM17_Hong_Kong_UCCKE   (2017-08-11)


Mammalian version of urate oxidase (UOX)


This is a subpart of BBa_K2197400. The part expresses smUOX, a mammalian version of urate oxidase. In the human purine and uric acid pathway, purine is converted to uric acid through a series of steps in the purine pathway. Uric acid forms crystals when high concentration is accumulated. Urate oxidase breaks down uric acid into allantoin so that the crystals can be excreted.

Design of BBa_K2197400
T--Hong_Kong_UCCKE--400gene2.jpg

Expression of a strong repressor (mUTS) T--Hong_Kong_UCCKE--Krab.jpg

Operator site and smUOX T--Hong_Kong_UCCKE--_400bindsite.jpg

Expression without uric acid (-UA) T--Hong_Kong_UCCKE--_400noua.jpg

mUTS binds to HucO and repress the expression of downstream smUOX.

expression with uric acid (+UA) T--Hong_Kong_UCCKE--wifUA400.jpg

mUTS dissociates with HucO to different extents according to the concentration of uric acid, thus expresses downstream smUOX at different levels.

Kemmer, Christian & Gitzinger, Marc & Daoud-El Baba, Marie & Djonov, Valentin & Stelling, Jörg & Fussenegger, Martin. (2010). Self-sufficient control of urate homeostasis in mice by a synthetic circuit. Nature biotechnology. 28. 355-60. 10.1038/nbt.1617.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 643
    Illegal PstI site found at 865
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 736
    Illegal PstI site found at 643
    Illegal PstI site found at 865
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 643
    Illegal PstI site found at 865
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 643
    Illegal PstI site found at 865
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 4
    Illegal SapI.rc site found at 954


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Categories
Parameters
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