Coding

Part:BBa_K2196005

Designed by: Julianna Casella, Nicholas Roig, Chloe Savino, Jerin Thomas, Megan Schiesser, and Timothy Darby   Group: iGEM17_Stony_Brook   (2017-10-23)


Lacticin Z Lacticin Q Hybrid

This part was synthesized by Integrated DNA Technologies. The coding sequence of this part was codon-optimized for production in E.coli using the proprietary codon optimization program on the Integrated DNA Technologies website. It codes for the hybrid bacteriocin Lacticin Q and Lacticin Z, joined by a triglycine linkage. They were joined at the N-terminus of Lacticin Q and the C-terminus of Lacticin Z. Since both of the bacteriocins are effective against MRSA and other Staphylococcus aureus strains, it is possible that the hybridization of the two will have some antimicrobial properties too. This part is designed to be inserted into a vector containing an RBS and a protein tag, so that it can be expressed in E. coli, cleaved from a protein tag, and purified. We hope that teams will be able to test the antimicrobial properties of this hybrid bacteriocin compared to the individual bacteriocins on their own.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 260
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None