Coding

Part:BBa_K2196004

Designed by: Nicholas Roig, Julianna Casella, Chloe Savino, Jerin Thomas, Megan Schiesser, and Timothy Darby   Group: iGEM17_Stony_Brook   (2017-10-23)


Lacticin Z Epidermicin NI01 Hybrid


This part was synthesized by Integrated DNA Technologies. It codes for the hybrid bacteriocin Epidermicin NI01 and Lacticin Z, joined by a triglycine linkage. The bacteriocins were joined at the N-terminus of Epidericin NI01 and C-terminus of the Lacticin Z. Since both of the bacteriocins are effective against MRSA and other Staphylococcus aureus strains, it is possible that the hybridization of the two will have some antimicrobial properties as well. This part is designed to be inserted into a vector containing an RBS and a protein tag, so that it can be expressed in E. coli, cleaved from a protein tag, and purified. We hope that teams will be able to test the antimicrobial properties of this hybrid bacteriocin compared to the individual bacteriocins on their own.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None