Coding

Part:BBa_K2196000

Designed by: Nicholas Roig, Julianna Casella, Chloe Savino, Jerin Thomas, Megan Schiesser, Timothy Darby   Group: iGEM17_Stony_Brook   (2017-10-23)


Aureocin A53

This part was synthesized by Integrated DNA Technologies and contains the coding sequence for the bacteriocin Aureocin A53. Aureocin A53 is synthesized by Staphylococcus aureus A53 and has antimicrobial properties towards MRSA and S. aureus strains. This part is designed to be inserted into a vector containing an RBS and a protein tag, so that it can be expressed in E. coli, cleaved from a protein tag, and purified. We hope that it will be beneficial to use this part as a possible antimicrobial agent against other pathogens.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None