Composite

Part:BBa_K2187001

Designed by: Ella Coleman   Group: iGEM16_Baltimore_BioCrew   (2016-10-14)


Chlorogenate Esterase gene

The chlorogenate esterase gene comes from the Ideonella sakaiensis bacteria. Its purpose is to create an enzyme called PETase which breaks down PET into MHET.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 69
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 69
    Illegal NotI site found at 75
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 69
    Illegal BamHI site found at 237
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 69
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 69
    Illegal XbaI site found at 84
    Illegal NgoMIV site found at 524
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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