Part:BBa_K2187000
Lipase gene
The Lipase gene comes from the Ideonella sakaiensis bacteria. Its purpose is to create and enzyme called MHETase that breaks down MHET into ethylene glycol and terephthelic acid.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 634
Illegal XhoI site found at 697 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
We are evaluating this part by verifying that it can be used to degrade PET. We are setting up an experiment in which we will test for the the ability of E.coli bacteria engineered with this part to degrade PET plastic. Glass culture tubes were set up containing LB media and small squares of PET plastic that had been weighed beforehand. The approximate dimensions of the plastic squares were 20x15x0.5mm, and they weighed between 101 and 89 mg. After the bacteria with the gene have been added, the plastic will be weighed twice a week to check for lost material. Some tubes will be incubated at 30 degrees Celsius because that is the best growth temperature for the Ideonealla bacteria, and some will be incubated at 37 degrees Celsius because that is the best temperature for E. coli.
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